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. 2021 Jul 20;11:706618. doi: 10.3389/fcimb.2021.706618

Figure 2.

Figure 2

F6 fraction from TsV triggers the production of pro-inflammatory mediators and induces macrophage anti-protozoal activity. TsV was separated by gel filtration chromatography in which the components were eluted at a flow rate of 0.5 mL/min and protein detection was performed at 280 nm. The fractions obtained were listed from 1–7 (A) Peritoneal macrophages were cultured and stimulated with F6 or F7 fractions (100 or 50 µg/mL, respectively), and at 48 h post-stimulation the culture supernatants were collected for nitric oxide measurement (B). Viability assay (MTT) was performed on the adhering cells at 48 h after F6 (100 µg/mL) (C) or F7 (50 µg/mL) stimulation (D). The cells were infected (1:1 parasite: cell ration) and/or stimulated with F6 (100 µg/mL) or (F7 50 µg/mL) and the culture supernatants were collected at 24 and 48 h post-stimulus; (E) F6 and (F) F7, for cytokine measurements (IL-12p70, TNF, and IL-6). Each point represents means ± SEM of one of the two independent experiments, *p ≤ 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.