Fig. 8.

PDIA6 inhibits the ubiquitination degradation of β-catenin and PD-L1 by up-regulating CSN5 expression. (A) Co-IP and Western blot were used to detect the ubiquitination levels of β-catenin and PD-L1 after the knockdown or overexpression of PDIA6. (B) The ubiquitination levels of β-catenin and PD-L1 were detected in PDIA6-overexpressing cells transfected with sh-CSN5. (C) Co-IP assay and Western blot were conducted to detect the relationship between PDIA6 and CSN5. (D) GST-pull down assay and Western blot was performed to determine the interaction between PDIA6 and CSN5. (E) CSN5 protein levels were assessed through western blot experiments after the downregulation or overexpression of PDIA6. N = 3. **P < 0.01, and ***P < 0.001. Data were mean ± SD. Unpaired Student's t test and one-way ANOVA with Tukey's post-tests.