Fig. 9.

PDIA6 promotes the development of PC by up-regulating CSN5 expression. (A) Relative protein levels of p-β-catenin (Ser33/Ser37/Thr41), β-catenin, and PD-L1 in BxPC-3 cells after the overexpression of CSN5. (B) Co-IP combined with Western blot was performed to measure the ubiquitination levels of β-catenin and PD-L1 in CSN5-overexpressing cells. (C-D) The cell viability, migration, and invasion of BxPC-3 cells were detected after the PDIA6-overexpressing cells were treated with or without CSN5 shRNA. (E) The protein levels of p-β-catenin (Ser33/Ser37/Thr41), β-catenin, and PD-L1 were measured by Western blot in the PDIA6-overexpressing cells treated with or without CSN5 shRNA. N = 3. *P < 0.05, **P < 0.01, and ***P < 0.001. ^#P < 0.05 and ^###P < 0.001. Data were mean ± SD. Unpaired Student's t test and one-way ANOVA and two-way ANOVA with Tukey's post-tests.