Table 1.

Applied detergent- and enzyme-based decellularization protocols

Protocol	1. step	2. step	3. step	4. step	Ref.
SDS/SD	4× 12 h	2× 12 h	14× 12 h		[6, 24]
	0.5% SDS/0.5% SD	H2O	PBS
TX + SDS	2× 12 h	2× 12 h	2× 12 h	14× 12 h	[21]
	0.5% TritonX-100	0.5% SDS	H2O	PBS
T+TX	90 min	4× 12 h	2× 12 h	14× 12 h	[21]
	0.125% Trypsin/0.05% EDTA	0.5% TritonX-100	H2O	PBS