Fig. 9.

ROS enhancement with the GAP for Cdc42. The indicated strains were induced to form germ tubes and subjected to dark field microscopy (A, top) and for assays of Fre8 ROS by luminol chemiluminescence (A, bottom and B) and for FRE8 mRNA (C) as described for Figure 6. The bar in part A represents 10 microns. Data represent five (B) or seven cultures (C) from three experimental trials. The bar represents mean; error bars represent SD. ∗∗∗p = 0.0001 as determined by two-tailed t test. The following strains were utilized: WT, BWP17; rga2 bem3Δ, 615. GAP, GTPase-activating protein; ns, not significant; ROS, reactive oxygen species.