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. 2021 Jul 20;12:661109. doi: 10.3389/fgene.2021.661109

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Copyright © 2021 Wang, Zou, Zhang, Li, Wang, Ke, Chen, Wang, Wang, Xu, Xie and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Procedure to validate the trained model using the external dataset with BRCA-mutation. (A) The WSI with BRCA-mutation. (B) The binary mask (top) was created using color differences in the RGB color space and then element-wise matrix multiplication was done with the manually labeled lesion (bottom) to create a lesion mask without void spaces. (C) Tiles with a resolution of 256 × 256 were extracted from the lesion mask. (D) Heatmap which illustrates the probability of the region being classified as BRCA-mutation.