FIGURE 2.

Representative images of sections of the nasal mucosa taken 30 and 90 days after mucosal resection (lower magnification, 40×; higher magnification, 200×). Hematoxylin and eosin (H&E) staining, Periodic Acid-Schiff and Alcian Blue (PAS/AB) staining, and Elastica van Gieson (EVG) staining are shown. (A) H&E staining. On day 30, the mucosa of the nasal septum (NS: a), including the olfactory epithelium (OE) and lamina propria (LP), had regenerated to some extent. The repaired OE consisted of stratified ciliated columnar cells, and the LP consisted of olfactory nerve bundles (star), connective tissue, and vessels. In the dorsolateral area (DL: b), the drilled hole had closed with the development of a new bone and connective tissue. On day 90, the OE in the NS had thinned, and its appearance had changed. The area occupied by olfactory nerve bundles was diminished. In the DL, the new osseous tissue had proliferated excessively and become covered by ciliated pseudostratified columnar epithelium. (B) PAS/AB staining revealed the presence of Bowman’s glands in the LP (red arrowheads). (C) EVG staining was used to highlight connective tissue, such as collagen fibers (stained red: black arrows).