Skip to main content
. 2021 Jul 20;12:689607. doi: 10.3389/fmicb.2021.689607

FIGURE 1.

FIGURE 1

The anti-HSV-1 activity of Oleanolic acid. (A) Chemical structure of Oleanolic acid. (B) Simple diagram of the performed experiments. (C,D) Vero cells were infected with HSV-1 (MOI = 1) in the presence of Oleanolic acid or ACV at different concentrations for 72 h. The cells were then fixed and stained with crystal violet dye. The plaque numbers were counted to calculate the inhibition rate. Data are mean ± SD (n = 2). (E) HaCaT cells were treated with HSV-1 (MOI = 1) and Oleanolic acid for 24 h. Viral DNA was extracted and the DNA copy number of viral gene α27 and UL47 were determined by qRT-PCR. Data are mean ± SD (n = 3). **p < 0.01, ***p < 0.001. (F,G) HaCaT cells were treated with HSV-1 (MOI = 1) and Oleanolic acid for 12 or 24 h, respectively. Virus titration (F) and the expression levels of viral protein ICP0, gB, and gD (G) were detected. Data are mean ± SD (n = 3). *p < 0.5. (H,I) SH-SY5Y and BV2 cells were infected with HSV-1 (MOI = 1) in the presence or absence of Oleanolic acid (OC) at indicated concentrations for 24 h. Total viral DNA was extracted and the DNA copy numbers of viral genes α0, UL27, and UL49 were determined by qRT-PCR (H). The supernatant and cell pellets were collected to calculate virus titers (I). Data are mean ± SD (n = 3). *p < 0.5, **p < 0.01, ***p < 0.001.