FIGURE 3.

Oleanolic acid affects viral early infection. (A) Simple diagram of the time-of-addition assay. (B) HaCaT cells infected with HSV-1 (MOI = 1) at 4°C for 2 h were washed with PBS and incubated at 37°C. Oleanolic acid were added at indicated time points. After 24 h, total viral DNA was extracted and the DNA copy numbers of viral gene α0 and UL47 were detected by qRT-PCR. (C,D) HaCaT and Vero cells were infected with HSV-1 (MOI = 1) at 4°C for 2 h, and were washed with PBS to remove the virions. The cells were then incubated at 37°C and were treated with Oleanolic acid at indicated time points. After 72 h, the supernatant of cell culture was collected to perform viral titration assay and calculate viral titers. Data are mean ± SD (n = 3).