FIGURE 4.

Oleanolic acid inhibits the expression of genes related to the HSV-1 helicase-primase complex. (A–C) HaCaT cells were treated with HSV-1 (MOI = 5) and Oleanolic acid (20 μM) for 2 and 4 h. Total RNA was extracted and the mRNA expression levels of gene UL5, UL8, and UL52 were detected by RT-qPCR. Data are mean ± SD (n = 3). *p < 0.5, **p < 0.01, ***p < 0.001. (D) The cells were transfected with overexpression plasmid Flag-UL5, HA-UL8, and EGFP-UL52 for 24 h and were infected with HSV-1 (MOI = 5) in the presence or absence of Oleanolic acid (20 μM) for 4 h. Cell lysates were collected and subjected to immunoprecipitation with anti-HA antibody and protein A/G-agarose. Immunoprecipitates were detected by western blot assay with anti-GFP, anti-HA and anti-Flag antibody.