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. 2021 Jul 20;12:677631. doi: 10.3389/fpls.2021.677631

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Copyright © 2021 McCaghey, Shao, Kurcezewski, Lindstrom, Ranjan, Whitham, Conley, Williams, Smith and Kabbage.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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External RNAs are taken up by Sclerotinia sclerotiorum. Treatments were added to potato dextrose broth (PDB) containing 2 day old S. sclerotiorum mycelia originally grown on potato dextrose agar (PDA). Fluorescent signals were detected using confocal fluorescent microscopy. (A) An application of water, as a negative control, yielded no signal. (B) The positive control, application of Cy3-labeled-nocoding siRNA (Cy3-NC-siRNA, with no specific targets in S. sclerotiorum), (C) 4 μg application Cy3-labeled-Ssoah1-dsRNA, and (D) application of 4 μg of Cy3-labeled-Ssoah1-siRNA (targeting Ssoah1 in S. sclerotiorum) produced fluorescent signals that were detected 13 h post inoculation. Scale bars = 20 μm.