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. Author manuscript; available in PMC: 2021 Aug 3.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2020 Oct 24;109(4):1040–1053. doi: 10.1016/j.ijrobp.2020.10.025

Figure 3. Benzodiazepine potentiates radiation.

Figure 3.

(A) Top, Schematic showing therapeutic strategy. Mice were implanted in left (L) and right (R) flanks with B16F10-GP tumor cells. Mice received either: (i) vehicle alone control; (ii) radiation alone (5 Gy, right-flank only); (iii) QH-II-066 beginning Day 10; (iv) radiation (5 Gy, right-flank only) on Day 10 (morning), followed by QH-II-066 (evening). Bottom, Effect of combination of QH-II-066 and 5 Gy on tumor growth kinetics. Tumor measurements are combination of two experiments; where, vehicle alone control, n=8; 5 Gy, n=9; QH-II-066, n=8; QH-II-066+5 Gy, n=9. In groups receiving QH-II-066, 10 mg/kg was injected i.p. daily for 7 days. (B) Percent CD8 of total lymphocytes and number of CD8 T cells per gram of tumor. TILs were isolated at day 7–8 after radiation. Error bars are representation of SEM. (C) Top, Representative flow showing percent IFN-γ and TNF-α in total CD8+ T cells after TILs were stimulated with peptide. Middle, Graph showing percent IFN-γ+CD8+ of total CD8+ T cells and number of IFN-γ+CD8 T+ cells per gram of tumor. Bottom, Graph showing percent TNF-α +CD8+ of total CD8+ T cells and number of TNF-α +CD8 T+ cells per gram of tumor. (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001). Abbreviation: i.p., intraperitoneal; TILs, Tumor infiltrating lymphocytes; IFN-γ, interferon gamma cytokine; TNF-α, Tumor necrosis factor alpha cytokine.