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. 2021 Jul 20;12:707728. doi: 10.3389/fimmu.2021.707728

Figure 4.

Figure 4

Untreated and heat-treated bifidobacteria strains influence the OVA-induced cytokine production in splenocyte cultures from OVA-sensitized mice. Effect of untreated (UN, blue bars) and heat-treated (HT, orange bars) bifidobacteria strains on cytokine production: (A) IL-5, (B) IL-4, (C) IL-13, and (D) IFN-γ was measured in supernatants after 72 h cultivation of splenocytes isolated from OVA-sensitized mice and re-stimulated by OVA with or without bifidobacteria strains. Cells stimulated by PBS only served as negative control. Values are pooled from two independent experiments. Significant difference between UN and HT bacteria was estimated by unpaired Student’s t-test (*p <.0.05). Comparison between OVA-stimulated and bifidobacteria + OVA-stimulated groups was calculated by one-way ANOVA Dunett’s multiple comparison test. OVA group was used as control, #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001.