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. 2021 Jul 20;15:690571. doi: 10.3389/fnbeh.2021.690571

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Copyright © 2021 Paffhausen, Petrasch, Wild, Meurers, Schülke, Polster, Fuchs, Drexler, Kuriatnyk, Menzel and Landgraf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Preparation setup and recording site. (A) Preparations for electrophysiological experiments were performed in a controlled environment with the honey bee already attached to the quadcopter. (B) A small rectangular incision into the head capsule revealed the bee brain's alpha lobe after the trachea and glands were pushed aside. The electrode bundle visible in the microscope was attached to a micromanipulator with dental wax. Once the electrode bundle was implanted, it was connected to the head stage on the copter via a custom connector jack. Finally, the electrodes and incision were sealed with silicone. Before the animal was moved to the field, the electrode holder was detached to allow for a free anterior view.