Fig. 1.

Erastin treatment promoted ferroptosis of primary spinal cord neurons. Primary neurons of the spinal cord were treated with different concentrations of Erastin (0, 1, 5, 10 μm) for 24 h. (A) CCK8 was performed to assess cell viability of primary spinal cord neurons. Student’s t‐test; the error bars indicate SD; n = 3. Primary spinal cord neurons were treated with Erastin (5 μm) with or without Fer‐1 (1 μm) for 24 h. Normal primary spinal cord neurons served as control. (B) CCK8 was performed to detect cell viability of primary spinal cord neurons. One‐way ANOVA; the error bars indicate SD; n = 3. (C) Cell morphology of primary spinal cord neurons was observed. Scale bar: 100 µm. **P < 0.01 vs. 0 or Ctrl group; ^## P < 0.01 vs. Erastin group.