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. 2021 Jul 8;11(8):2118–2126. doi: 10.1002/2211-5463.13203

Fig. 3.

Fig. 3

LXA4 inhibited Erastin‐induced ferroptosis of primary spinal cord neurons through the AKT/Nrf2/HO‐1 signaling pathway. Primary spinal cord neurons were treated with Erastin (5 μm) with or without LXA4 (100 nm) for 24 h. Normal primary spinal cord neurons served as control. (A) WB was performed to examine the expression of p‐AKT, AKT, Nrf2 and HO‐1 in the primary spinal cord neurons. One‐way ANOVA; the error bars indicate SD; n = 3. Primary spinal cord neurons were treated with Erastin (5 μm) with or without LXA4 (100 nm) for 24 h, and then treated with LY294002 (10 μm) for 1 h, BT (20 nm) for 2 h or ZnPP (20 μm) for 2 h. Primary spinal cord neurons were treated with Erastin (5 μm) as control. (B) WB was performed to examine the expression of GPX4, PTGS2 and ACSL4 in the primary spinal cord neurons. One‐way ANOVA; the error bars indicate SD; n = 3. (C) DCFH‐DA probe was used to detect the levels of ROS in the primary spinal cord neurons. One‐way ANOVA; the error bars indicate SD; n = 3. Scale bars: 100 µm. **P < 0.01 vs. control (Ctrl) group; # P < 0.05, ## P < 0.01 vs. Erastin group; $$ P < 0.01 vs. LXA4 + DMSO group.