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Knockdown of USP7 or USP10 decreased NHE3 abundance. The effect of USP7 (A) or USP10 (B) knockdown on NHE3 activity was determined in Caco-2bbe/NHE3 cells. NHE3 activity was determined as Na+-dependent intracellular pH recovery. Results are presented as mean ± SEM. n ≥ 10. *, P < .05, **, P < .01 compared with shCon
