Table 3.

In vitro sperm quality parameters assessed on frozen-thawed semen doses by CASA and FCM of 10 Holstein bulls based on fertility ranking in the CG and HQS fraction (LSM ± SEM).

Fraction	CG				HQS Fraction
	HF		LF		HF		LF
Parameters	LSM	SEM	LSM	SEM	LSM	SEM	LSM	SEM
MOT TOT	83.27a	4.00	63.02b	4.00	83.96	4.66	78.04	4.66
PROG	54.49a	3.07	43.80b	3.07	52.35	4.12	52.64	4.12
VSL	54.01	3.31	52.57	3.31	60.77	6.29	64.83	6.29
VCL	90.19	4.49	82.33	4.49	111.95	6.49	112.60	6.49
VAP	64.34	3.45	59.53	3.45	75.18	6.46	76.78	6.46
LIN	59.76	2.58	64.36	2.58	53.28	3.74	57.23	3.74
STR	83.73a	1.38	88.09b	1.30	79.51	1.97	84.11	1.97
WOB	71.16	2.13	72.85	2.13	66.27	3.13	67.73	3.13
ALH	2.96	0.14	2.73	0.14	3.69	0.18	3.65	0.18
BCF	9.85	0.38	10.25	0.38	8.48a	0.34	9.58b	0.34
VIAB	48.73	4.26	40.07	4.26	66.89	2.97	62.42	3.14
VIA	28.12	2.66	22.08	2.66	36.82	4.04	42.11	4.27
VDA	1.88	0.25	2.04	0.25	1.54	0.17	1.70	0.18
ALPHA-T	0.4097a	0.0016	0.4150b	0.0016	0.4025a	0.0020	0.4119b	0.0020
ATSD	0.0093	0.0009	0.0112	0.0009	0.0113	0.0006	0.0124	0.0006
%DFI	1.3337	0.3215	1.8830	0.3215	0.7327	0.2314	1.1106	0.2314
%HG	1.5775	0.2210	2.1160	0.2210	2.0627	0.1672	2.0502	0.1672

MOT TOT, total motility; PROG, cells progressive motility; VSL, straight-line velocity; VCL, curvilinear velocity; VAP, average path velocity; LIN, linear coefficient; STR, straightness coefficient; WOB, wobble coefficient; ALH, amplitude of lateral head displacement, BCF, beat cross-frequency; VIAB, viable sperm; VIA, viable with intact acrosome; VDA, viable with disrupted acrosome; ALPHA-T, red/(red + green) fluorescence intensity; ATSD, Alpha-T standard deviation; %DFI, fragmented DNA sperm; %HG, high green fluorescence sperm.

^a,bvalues within a row with different superscripts differ significantly at P < 0.05.