Figure 6.

DWV and FHV infection transferred from infected to naïve honey bee pupal cells. To confirm that infectious DWV and FHV virions were produced in infected primary honey bee pupal cells, mock- or virus-infected honey bee pupal cell cultures (10 µL) at 72 hpi were transferred to naïve pupal cells. Virus abundance was assessed over a time course (i.e., 0 hpi, 48 hpi, and 72 hpi) using qPCR. Raw data are included in Supplemental Tables S7 and S8. (A) FHV abundance increased 15.8× at 48 hpi (p = 0.003), with a subsequent 28× increase at 72 hpi relative to 0 hpi (p < 0.001). (B) DWV abundance was 2.7× (p < 0.001) higher at 48 hpi and 4.1× higher at 72 hpi, relative to 0 hpi (p < 0.001). Figure 6 includes results from one representative biological replicate. The data for additional replicates are presented in Supplemental Figure S6. All differences in means relative to 0 hpi were assessed by a Dunnett’s test. Significance levels: ** p < 0.005; *** p < 0.0005.