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. 2021 Aug 3;19:119. doi: 10.1186/s12958-021-00773-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TUNEL assay of DNA fragmentation in arrested 2–4 cells. A Column a) is the nuclear counterstain (Hoechst), column b) is the FITC-labelled DNA fragmentation, column c) is an overlay of both. The last two rows display the negative and positive controls of the TUNEL assay. B Average DNA fragmentation in arrested 2–4 cells via TUNEL assay. Percentage of DNA fragmentation in each embryo determined as a ratio of the number of cells with DNA fragmentation to the total number of cells in the arrested embryos. * represents statistical significance p < 0.05 and error bars represent +/− SEM (n = 15 embryos per group)