Table 1.
TEER values and paracellular mannitol permeability (basolateral to apical) in nm/s and percentage of transport per hour of RBE4 and hCMEC/D3 cells were measured 7 days after seeding
| Cell type | TEER | Mannitol permeability | |
|---|---|---|---|
| (Ω cm2) | (nm/s) | (%/h) | |
| RBE4-WT | 15.55 ± 1.22 | 59.58 ± 4.07 | 4.20 ± 0.30 |
| RBE4-MDR1 + dox | 12.63 ± 0.54 | 60.69 ± 1.59 | 4.30 ± 0.22* |
| hCMEC/D3-WT | 16.57 ± 1.06 | 53.76 ± 4.32 | 3.71 ± 0.13 |
| hCMEC/D3-MDR1 + dox | 15.79 ± 1.18 | 56.65 ± 2.52 | 3.81 ± 0.11 |
Data are presented as the mean ± SEM of 3–8 replicates. Experiments were performed with ThinCert® cell culture inserts (12-well, 0.4 µm pore size, PET membranes). See “Materials and methods” and Additional file 1 for further details. Significant differences as calculated by one-way ANOVA (Bonferroni post hoc test) compared to hCMEC/D3-WT are marked by asterisk. All other values were not significantly different (P < 0.05)