Figure 6.

In vitro conditions are placed on the platforms to demonstrate their ability to maintain fluorescence intensity and support cell growth. (a) Biotinylated SWNT and non-biotinylated SWNT (30 mg/L) were applied to avidin and APTES substrates, respectively. Slides were stored in individual slide holders at 37 °C with 4 mL of L-15 media, which was replaced daily. Fluorescence intensity values were tracked over the course of 10 days (n = 3, s = 5). The avidin + biotinylated SWNT sample was found to consistently outperform the APTES + non-biotinylated sample with more than triple its fluorescence intensity. (b) Cell growth is shown for breast cancer cells (MDA MB 231) that were seeded on the sensor platforms. Images of cells were taken at 12, 24, 48, and 72 h time points, demonstrating cell growth over a three day time frame.