Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 29;12:20417314211021679. doi: 10.1177/20417314211021679

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 3. — LRG1 is a target of miR-129-5p. (a) Venn diagram of the target genes of miR-129-5p predicted by the starBase and mirDIP databases, and upregulated genes in the GSE124272 dataset; expression of LRG1 in IDD in the GSE124272 dataset. (b) Putative miR-129-5p binding sites in the 3′UTR of LRG1 mRNA in the starBase website. (c) Expression of LRG1 detected by RT-qPCR in normal and NP tissues of 30 IDD patients. (d) Pearson correlation analysis of miR-129-5p expression with LRG1 in NP tissues. (e) Binding of miR-129-5p to LRG1 verified by dual-luciferase reporter assay in HEK-293T cells. (f) Expression of LRG1 detected by RT-qPCR in NP cells transfected with miR-129-5p mimic or inhibitor. (g) Western blot analysis of LRG1 protein in NP cells transfected with miR-129-5p mimic or inhibitor. *p < 0.05. The experiment was run in triplicate independently.