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. 2021 Jul 29;12:20417314211021679. doi: 10.1177/20417314211021679

Figure 4.

Figure 4.

LRG1 facilitates cell apoptosis, ECM degradation, and M1 polarization of macrophages by activating the p38 MAPK signaling pathway in NP cells. (a) p38 phosphorylation level determined by Western blot analysis in normal and NP tissues of 30 IDD patients. (b) LRG1 mRNA expression detected by RT-qPCR in IL-1β-induced NP cells treated with sh-LRG1. (c) p38 phosphorylation level determined by Western blot analysis in IL-1β-induced NP cells treated with sh-LRG1. IL-1β-induced NP cells were treated with oe-LRG1 or in combination with SB202190. (d) LRG1 mRNA expression detected by RT-qPCR in NP cells. (e) Viability of NP cells detected by CCK-8 assay. (f) Apoptosis of NP cells detected by flow cytometry. (g) Flow cytometric analysis of expression of M1 marker CD86 and M2 marker CD206 in RAW264.7 cells. (h) Expression of TNF-α, IL-6, IL-4, and IL-10 detected by ELISA in RAW264.7 cells. *p < 0.05. The experiment was run in triplicate independently.