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. Author manuscript; available in PMC: 2021 Aug 3.
Published in final edited form as: J Vis Exp. 2019 May 12;(147):10.3791/58992. doi: 10.3791/58992

Figure 3: Flow and optical changes over time.

Figure 3:

The optical density change (ΔOD) of each chromophore is simply the fitted individual chromophore’s spectrum at its maximum absorbance. The maximum absorbance frequencies were as previously described20,26. The presented time course is for one experiment, showing a baseline, followed by cyanide injection (0.10 mM at maximum perfusate flow), cyanide washout, nitrogen hypoxia performed by replacing oxygen with nitrogen, and then complete ischemia. Please click here to view a larger version of this figure.