Figure 2.

Knockdown of RACK1 induces senescence of lung squamous cell carcinoma cells. (A) RACK1 expression in NCI-H226 and H1703 cells was detected. (B) RACK1 was knocked down in NCI-H226 and H1703 cells, and anchorage-independent growth was detected with colony formation assays. (C) β-gal staining was used to detect cell senescence. (D) RACK1 expression was knocked down in NCI-H226 and H1703 cells, subcutaneous tumorigenesis assays were performed, and the tumor weights were statistically analyzed. (E) Immunohistochemistry was used to detect the expression of Ki67 in tumors derived from RACK1 knockdown NCI-H226 cells (20x). Left: representative immunohistochemistry image; right: RACK1 and MDM2 protein levels in tumors. **P < 0.01.