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. 2021 Aug 15;18(3):750–762. doi: 10.20892/j.issn.2095-3941.2020.0566

Figure 2.

Figure 2

Figure 2

Raltitrexed has the highest hyperthermia chemotherapy sensitization enhancement ratio score among the screened chemotherapy drugs. (A) The dose-response curves of organoids derived from patient 1 after treatment with different drugs for 90 min at 37 °C and 43 °C. Cell viability at 37 °C was measured with 10% CCK-8 after 2 days under drug-free conditions after drug treatment. The hyperthermia chemotherapy sensitization enhancement ratio (HCSER) score was obtained by calculating the ratio of the IC50 values between 37 °C and 43 °C for each drug. That is, HCSER = IC50 (37 °C)/IC50 (43 °C). (B) The HCSER scores of organoids derived from the other 10 patients. Raltitrexed had the highest HCSER score. (C) The highest HCSER scores of the 7 chemotherapy drugs in organoids from 22 patients. (D) Dose-response curves of organoids derived from DLD-1 cell lines, and the HCSER summary for all evaluated drugs. (E) The HCSER scores of the organoids derived from another 2 colorectal cancer cell lines. Raltitrexed showed the highest HCSER scores. (F) Counts of the highest HCSER scores of the 7 chemotherapy drugs in the 4 colorectal cancer cell lines.