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. 2021 Aug 15;18(3):675–692. doi: 10.20892/j.issn.2095-3941.2020.0136

Figure 2.

Figure 2

DPP10-AS1 promotes lung cancer cell proliferation in vitro and tumor growth in vitro. MTT assays were performed to determine the viability of lung cancer cells treated with siDPP10-AS1 in SPC-A1 (A) and NCI-H1299 (B) cells, and with pcDNA3.1-DPP10-AS1 in SPC-A1 (C) and NCI-H1299 (D) cells. Colony formation assays were used to detect the proliferation ability of lung cancer cells after transfection with siDPP10-AS1 in SPC-A1 (E) and NCI-H1299 (F) cells, and pcDNA3.1-DPP10-AS1 in SPC-A1 (G) and NCI-H1299 (H) cells. Lung cancer cells overexpressing pcDNA3.1-DPP10-AS1 were injected subcutaneously into nude mice to demonstrate xenograft tumor growth (I). Analysis of tumor volume (J) and tumor weight (K) in SPC-A1 cells with overexpression of pcDNA3.1-DPP10-AS1. Analysis of tumor volume (L) and tumor weight (M) in NCI-H1299 cells with overexpression of pcDNA3.1-DPP10-AS1. RT-qPCR analysis of lncRNA DPP10-AS1 (N) and DPP10 mRNA (O) expression in tumor tissues after injection of SPC-A1 cells. RT-qPCR analysis of lncRNA DPP10-AS1 (P) and DPP10 mRNA (Q) expression in tumor tissues after injection of NCI-H1299 cells. Western blot assays of DPP10 protein in tumor tissues overexpressing lncRNA DPP10-AS1 and empty plasmid (R). Colonies were counted and captured. The bar charts statistically compare the differences in colony formation in each experimental group compared with the corresponding control. Values are shown as the mean ± SD in 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.