Table 1.

Binding site comparisons between the HIF-1α PAS-B/VHH212 complex and HIF-1α PAS-B/ARNT PAS-B

PPI	HIF-1α and VHH212 complex				4H6J29
	VHH212		HIF-1α		ARNT		HIF-1α
	Position	Residue	Position	Residue	Position	Residue	Position	Residue
PPII	61	ASP	256	ASP	362	ARG	245	GLU
			257	GLU	379	ARG
			258	ARG	366	ARG	256	ASP
PPMMHB	109	GLY	337	CYS
	110	TYR	244	SER
PPAAI	110	TYR	254	TYR	375	PHE	254	TYR
PPMSHB	99	ARG	326	ASN	440	ARG	325	TYR
			327	THR
			328	LYS
PPSS	61	ASP	258	ARG	245	GLU	362	ARG
HB	110	TYR	256	ASP			379	ARG
PPHI	100	PRO	324	ILE	243	LEU	364	ILE
			325	TYR			375	PHE
	106	PHE	338	VAL			458	ILE
			340	TYR	254	TYR	364	ILE
			342	VAL			375	PHE

PPI, protein-protein interactions; PPII, protein-protein ionic interactions; PPMMHB, protein-protein main chain-main, chain hydrogen bonds; PPAAI, protein-protein, aromatic-aromatic interactions; PPMSHB, protein-protein, main chain-SIDE chain hydrogen bonds; PPSSHB, protein-protein side chain-side, chain hydrogen bonds; PPHI, protein-protein hydrophobic interactions.