Figure 4.

NEDD9 regulates CXCL8 expression by the ERK pathway. (A) Chemokine profiles were determined in the supernatants of NEDD9 knockdown and overexpression cells using the Human Multi-Cytokine assay. Heat map showing differential expression of genes between NEDD9 knockdown/overexpression and vector cells. The mRNA and protein expressions of CXCL8 were measured between NEDD9 knockdown and vector cells (B, C) or between NEDD9 overexpression and vector cells (D, E). (F) Activities of p-ERK1/2 (Thr202/Tyr204, 1:1,000), p-STAT3 (Tyr705, 1:1,000), and p-NF-κB (Ser536, 1:1,000) were measured by Western blot in NEDD9 knockdown and overexpression cells. (G, H) Expression of CXCL8 was determined between NEDD9 overexpression and vector cells with or without the ERK inhibitor (SCH772984). *P < 0.05; **P < 0.01; ***P < 0.001