Figure 2.

Profiling effects of CDNs on murine bone marrow derived MDSCs. (A) Summary of T cell suppression assay using BM-MDSCs stimulated with the indicated CDN for 24 hours at 2.5 µg/mL. CFSE dilution at the 1:1 MDSC:T cell ratio is shown, and per cent of live CD8 T cells proliferating at each indicated ratio is shown by heat map. For B–E, BM-MDSC were stimulated by indicated CDN at 2.5 µg/mL for 24 hours, followed by RNA, whole cell pellet, or supernatant harvesting for downstream multiomic analysis. (B) Gene set enrichment analysis results of RNA sequencing data represented as log₂ normalized pathway enrichment score for each condition as compared with untreated BM-MDSC. Focus on hallmark gene sets. (C) Same as in figure part B), focused on transcription factor targets gene sets. (D) Relative protein levels as measured by reverse phase protein array, following log₂ normalization and median centering of the data. (E) Secreted analyte concentrations in culture supernatants measured using Luminex multiplex cytokine/chemokine analysis. Data were manually segregated to display relevant low abundance and high abundance analytes. Data are cumulative of MDSCs from 4 to 8 murine donors in two independent batches. CDN, cyclic dinucleotide; MDSC, bone marrow derived MDSC.