Table 3.
Overview of immunological responses against E6 and E7 peptide pools
| Patient | Ex vivo | Clinical | E6-1 | E6-2 | E7 | |||||||||||
| Response | Response | IFNy | TNFa | IL2 | CD107a | IFNy | TNFa | IL2 | CD107a | IFNy | TNFa | IL2 | CD107a | |||
| 1 | Yes | CR | CD4 | D0 | <0.1% | 1.1 | <0.1% | 0.4 | <0.1% | 1.8 | 1.3 | 0.4 | <0.1% | 1.2 | 1.2 | 0.4 |
| D56 | <0.1% | 0.4 | 0.4 | 0.7 | 3.4 | 0.5 | 0.3 | 0.9 | 2.0 | 0.6 | 1.4 | 0.7 | ||||
| CD8 | D0 | <0.1% | n.d. | n.d | <0.1% | <0.1% | n.d. | n.d. | <0.1% | 1.5 | n.d. | n.d. | <0.1% | |||
| D56 | <0.1% | n.d. | <0.1% | <0.1% | 8.7 | n.d. | <0.1% | <0.1% | 3.8 | n.d. | 2.0 | 0.0 | ||||
| 2 | Yes | CR | CD4 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | 0.8 | <0.1% | <0.1% | <0.1% |
| D56 | 0.7 | <0.1% | <0.1% | 15.9 | 2.3 | <0.1% | <0.1% | 39.9 | 4.9 | <0.1% | <0.1% | <0.1% | ||||
| CD8 | D0 | 0.9 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | 0.2 | |||
| D56 | 0.8 | <0.1% | <0.1% | 9.5 | 1.7 | <0.1% | <0.1% | 5.9 | 3.4 | <0.1% | <0.1% | 6.3 | ||||
| 3 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | 1.1 | <0.1% | <0.1% | <0.1% | 1.0 | <0.1% |
| D56 | <0.1% | <0.1% | 1.0 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | 1.0 | <0.1% | ||||
| CD8 | D0 | 1.2 | <0.1% | 0.6 | 0.8 | 0.9 | <0.1% | 1.0 | 1.2 | 0.7 | <0.1% | 1.0 | 0.8 | |||
| D56 | <0.1% | <0.1% | 1.0 | 1.7 | <0.1% | <0.1% | 1.0 | 1.0 | <0.1% | <0.1% | 1.0 | 0.9 | ||||
| 4 | Yes | PR | CD4 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% |
| D56 | 10.1 | <0.1% | 1.6 | 1.8 | 6.4 | <0.1% | 0.9 | 1.2 | <0.1% | <0.1% | 4.0 | <0.1% | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | 1.2 | 1.2 | <0.1% | <0.1% | 1.1 | 0.9 | |||
| D56 | 24.2 | <0.1% | 3.6 | 1.0 | 12.4 | n.d. | 2.8 | 1.1 | <0.1% | <0.1% | 2.1 | 1.0 | ||||
| 5 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | <0.1% |
| D56 | <0.1% | <0.1% | <0.1% | 1.2 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | 0.7 | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | |||
| D56 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| 6 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% |
| D56 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | |||
| D56 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| 7 | Yes, | PR | CD4 | D0 | <0.1% | <0.1% | 5.2 | <0.1% | <0.1% | <0.1% | 6.0 | <0.1% | <0.1% | <0.1% | 2.4 | <0.1% |
| Vaccine enhanced, | D56 | 22.1 | <0.1% | <0.1% | <0.1% | 27.3 | <0.1% | <0.1% | <0.1% | 19.8 | <0.1% | <0.1% | <0.1% | |||
| not induced | CD8 | D0 | 13.8 | <0.1% | <0.1% | 1.2 | 5.3 | <0.1% | <0.1% | 0.8 | <0.1% | <0.1% | <0.1% | 0.8 | ||
| D56 | 6.4 | <0.1% | <0.1% | 0.7 | 7.0 | <0.1% | <0.1% | 1.7 | 6.3 | <0.1% | <0.1% | 0.8 | ||||
| 8 | Yes | PR | CD4 | D0 | <0.1% | <0.1% | <0.1% | 1.4 | <0.1% | <0.1% | <0.1% | 1.7 | <0.1% | <0.1% | <0.1% | 1.4 |
| D56 | <0.1% | <0.1% | 2.3 | 1.5 | 3.2 | <0.1% | 1.9 | 1.8 | <0.1% | <0.1% | 1.4 | 1.0 | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | 0.8 | 1.7 | <0.1% | <0.1% | 0.9 | 0.7 | <0.1% | <0.1% | 1.1 | |||
| D56 | 0.9 | <0.1% | 2.6 | 1.4 | 4.3 | <0.1% | 2.9 | 1.2 | 1.1 | <0.1% | <0.1% | 1.0 | ||||
| 9 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% |
| D56 | <0.1% | <0.1% | <0.1% | 1.0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | |||
| D56 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| 10 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | 0.6 | <0.1% | 1.1 | <0.1% | 1.1 | <0.1% | <0.1% | <0.1% | 0.7 |
| D56 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | 1.2 | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | 0.7 | <0.1% | <0.1% | <0.1% | 1.2 | <0.1% | <0.1% | <0.1% | 0.8 | |||
| D56 | <0.1% | <0.1% | <0.1% | 1.2 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | 1.1 | ||||
| 11 | No | NR | CD4 | D0 | <0.1% | <0.1% | 1.7 | <0.1% | <0.1% | <0.1% | 0.7 | <0.1% | <0.1% | <0.1% | 1.0 | 1.8 |
| D56 | <0.1% | <0.1% | <0.1% | 1.0 | <0.1% | <0.1% | 0.9 | 1.0 | <0.1% | <0.1% | 0.6 | 0.9 | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | 0.9 | 1.1 | <0.1% | <0.1% | 1.0 | 1.1 | <0.1% | <0.1% | 1.1 | |||
| D56 | 1.1 | <0.1% | <0.1% | 0.9 | 1.2 | <0.1% | <0.1% | 0.9 | 1.1 | <0.1% | <0.1% | 1.0 | ||||
| 12 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | 1.0 | 0.9 | <0.1% | <0.1% | 1.7 | 0.7 |
| D56 | <0.1% | <0.1% | <0.1% | 1.2 | <0.1% | <0.1% | 1.4 | 1.6 | <0.1% | <0.1% | 1.0 | 1.3 | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | 1.0 | <0.1% | <0.1% | <0.1% | 1.1 | <0.1% | <0.1% | <0.1% | <0.1% | |||
| D56 | 0.7 | <0.1% | <0.1% | 0.9 | 0.7 | <0.1% | 1.8 | 0.9 | 0.5 | <0.1% | <0.1% | 0.9 | ||||
| 13 | No | PR | CD4 | D0 | <0.1% | <0.1% | <0.1% | 1.8 | <0.1% | <0.1% | <0.1% | 1.8 | <0.1% | <0.1% | <0.1% | <0.1% |
| D56 | <0.1% | <0.1% | <0.1% | 1.9 | <0.1% | <0.1% | <0.1% | 1.7 | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| CD8 | D0 | <0.1% | <0.1% | <0.1% | 0.7 | <0.1% | 1.0 | <0.1% | 1.0 | <0.1% | <0.1% | <0.1% | <0.1% | |||
| D56 | <0.1% | 0.8 | <0.1% | 0.7 | <0.1% | 0.6 | <0.1% | 0.9 | <0.1% | <0.1% | <0.1% | 1.2 | ||||
| 14 | No | NR | CD4 | D0 | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% | <0.1% |
| D56 | <0.1% | <0.1% | <0.1% | 1.9 | <0.1% | <0.1% | <0.1% | 1.2 | <0.1% | <0.1% | <0.1% | <0.1% | ||||
| CD8 | D0 | <0.1% | <0.1% | 0.6 | 0.8 | <0.1% | <0.1% | 1.0 | 0.7 | <0.1% | <0.1% | <0.1% | <0.1% | |||
| D56 | <0.1% | <0.1% | <0.1% | 0.8 | <0.1% | <0.1% | <0.1% | 0.9 | <0.1% | <0.1% | 1.1 | 0.6 | ||||
Numbers represent the fold change over background (unloaded APCs). n.d.: no positive cells detected. Fold changes greater than two are highlighted in green. <0.1% indicates that the fraction of positive cells was less than 0.1% from its parent (CD4+ or CD8+ T cells).
CR, complete response; IFNγ, interferon gamma; IL2, interleukin 2; NR, no response; PR, partial response; TNFa, tumor necrosis factor alpha.