Figure 4.

Peptide P1 penetration comparison between penetration enhancer and peptide. (A) Fluorescence microscopy images of peptide P1 (5 μM) incubated with different penetration enhancers. (B) Quantification of fluorescent intensity of peptide P1 (5 μM) incubated with different penetration enhancers. The fluorescence of the cellular uptake was normalized by cellular protein. Values represent mean ± SEM. (C) The corresponding p-value plot between data pairs presenting in Figure 4(B). ANOVA was used to compare the differences between the control and experimental values. (D) Fluorescence microscopy images of peptide P1 (5 μM) and other published CPPs. (E) Quantification of fluorescent intensity of peptide P1 (5 μM) and other published CPPs. The error bars express SEM, the fluorescence of the cellular uptake was normalized by cellular protein. ANOVA was used to compare the differences between the control and experimental values, * indicated p < .05, and ** indicated p <.01.