Oct4 (169bp)	Forward: 5′-CTTGCTGCAGAAGTGGGTGGAGGAA-3′
	Reverse: 5′ -CTGCAGTGTGGGTTTCGGGCA-3′
GATA4 (194bp)	Forward: 5′-TCCCTCTTCCCTCCTCAAATTC-3′
	Reverse: 5′ -TCAGCGTGTAAAGGCATCTG-3′
Flk1 (400 bp)	Forward: 5′-CATATCTGTCCTGATGTGATATGTC-3′
	Reverse: 5′ -CATAGCATGTCTTATAGTCATTGTTC-3′
HBE1 (126 bp)	Forward: 5′-TGCATGTGGATCCTGAGAAC-3′
	Reverse: 5′ -CGACAGCAGACACCAGCTT-3′
Pax6 (317 bp)	Forward: 5′-ACCCATTATCCA-GATGTGTTTGCCCGAG-3′
	Reverse: 5′ -ATGGTGAAGCTGGGCATAGGCGGCAG-3′
T (274 bp)	Forward: 5′-GCCCTCTCCCTCCCCTCCACGCACAG-3′
	Reverse: 5′ -CGGCGCCGTTGCTCACAGACCACAGG-3′
E-cadherin (129 bp)	Forward: 5′-CCTGGTTCAGATCAAATCCAAC-3′
	Reverse: 5′ -GTCACCTTCAGCCATCCTG-3′
β-Actin (401 bp)	Forward: 5′-ACAGCAGTCGGTTGGAGCGAGCATC-3′
	Reverse: 5′ -CAAGTCAGTGTACAGGTAAGCCCTG-3′
Nodal (126 bp)	Forward: 5′-ACCGAGTCCCTTCCACTTGTTG-3′
	reverse: 5′-AGAGGCACCCACATTCTTCCAC-3′