Table 2.
List of primers
| Gene name | Sequence | Annealing temp [°C] |
|---|---|---|
| qPCR: | ||
| CRH | Forward:CACCCTCAGCCCTTGGATTTC | 57 |
| Reverse: GCCCTGGCCATTTCCAAGAC | ||
| POMC | Forward: GAGGGCAAGCGCTCCTACTCC | 63 |
| reverse: GGGGCCCTCGTCCTTCTTCTC | ||
| MC1R | Forward: ACTCACCCATGTACTGCTTC | 63 |
| Reverse: TACAGCACGGCCATGAGCAC | ||
| MC4R | Forward: CAGCATGGTGAAGAACATGG | 63 |
| Reverse: ATGACAGTTAAGCGGGTTGG | ||
| UCN1 | Forward: CAGGCGAGCGGCCGCG | 65 |
| Reverse: CTTGCCCACCGAGTCGAAT | ||
| UCN2 | Forward: GTGTCGGCCACTGCTGAGCCTGAGAGA | 65 |
| Reverse:ATCTGATATGACCTGCATGACAGTGGCT | ||
| UCN3 | Forward: TGCTGCTCCTGCTGCTGCTC | 58 |
| Reverse: GTGTCCTGGCGTGGCTTTCCC | ||
| NR3C1 | Forward: GAGACCAGATGTAAGCTCTCCT | 55 |
| Reverse: GCAATCATTCCTTCCAGCAC | ||
| RPL37A | Forward: TTCTGATGGCGGACTTTACC | 55 |
| Reverse: CACTTGCTCTTTCTGTGGCA | ||
| Nested PCR: | ||
| CRHR1 exon 2-7 round 1 | Forward:TCCGTCTCGTCAAGGCCCTTC | 60 |
| Reverse: AGTGGATGATGTTTCGCAGGCAC | ||
| CRHR1 exon 2-7 round 2 | Forward:TGTCCCTGGCCAGCAACATCTC | 60 |
| Reverse: GGCTCATGGTTAGCTGGACCAC | ||