Table 3.
List of antibodies used in the study
| Primary immunoglobulin | Manufacturer | Catalogue No. | Dilution | Secondary immunoglobulin/detection kit |
|---|---|---|---|---|
| Anti-CRH (Gt) | Santa Cruz | sc-21675 | 1 : 100 | VECTASTAIN® ABC HRP Kit (Peroxidase, Goat IgG) |
| Anti-CRHRl (Gt) | Santa Cruz | ac-12381 | 1 : 100 | VECTASTAIN® ABC HRP Kit (Peroxidase, Goat IgG) |
| Anti-ACTH (Rb) | courtesy of dr. Parlow, | 1 : 100 | ImmPRESS™ HRPAnti-Rabbit IgG (Peroxidase) Polymer Detection Kit | |
| Harbor-UCLA Medical Center | 1 : 100 | |||
| Anti-UCN1 (Rb) | Abcam | ab58459 | 1 : 100 | ImmPRESS™ HRPAnti-Rabbit IgG (Peroxidase) Polymer Detection Kit |
| Anti-MCIR (Rb) | Abcam | ab125031 | 1 : 100 | ImmPRESS™ HRPAnti-Rabbit IgG (Peroxidase) Polymer Detection Kit |
Gt – host organism: goat, Rb – host organism: rabbit.