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. Author manuscript; available in PMC: 2021 Oct 9.
Published in final edited form as: ACS Infect Dis. 2020 Sep 11;6(10):2629–2640. doi: 10.1021/acsinfecdis.0c00259

Figure 7.

Figure 7.

Variation in gene expression for the two component system phoPQ and the pmrCAB operon in K. pneumoniae B9 upon treatment with (A) 5 μM prodigiosin, (B) 1.25 μM prodigiosin with 2 μg/mL colistin, (C) 10 μM novobiocin, and (D) 2.5 μM novobiocin with 2 μg/mL colistin. All relative quantification (RQ) is calculated using the 12S rRNA rpsL as the reference gene. Error bars represent standard error of ΔΔCt values across 6 reactions in 2 independent samples. *p < 0.05 by Student’s t test comparing ΔCt of test reactions and control reactions, indicating statistically significant differences upon treatment with 5 μM prodigiosin.