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. 2021 Jun 21;20(13):1320–1333. doi: 10.1080/15384101.2021.1939476

Figure 4.

Figure 4.

MiR-326 overexpression partially reversed the effect of CircHIPK3 on the expression of apoptosis- and metastasis-related proteins in MCF7 cells. (a) Western blot detected the expressions of Bax, Bcl-2 and cleaved Caspase-3 in MCF7 cells transfected with miR-326 and /or circHIPK3. (b) RT-qPCR detected the expressions of Bax and Bcl-2 in MCF7 cells transfected with miR-326 and /or circHIPK3. (c) Western blot detected the expressions of E-Cadherin, N-Cadherin and Vimentin in MCF7 cells transfected with miR-326 and /or circHIPK3. (d) RT-qPCR indicated the expressions of E-Cadherin, N-Cadherin and Vimentin in MCF7 cells transfected with miR-326 and /or circHIPK3. GAPDH was used as an internal control. *P < 0.05 or **P < 0.01 or ***P < 0.001 vs. NC+MC; #P < 0.05 or ##P < 0.01 or ###P < 0.01 vs. CircHIPK3+ MC; ^P < 0.05 ^^P < 0.01 ^^^P < 0.001 vs. NC+M. Data are shown as mean±SD, n = 3. NC, negative control; MC, mimic control; M, miR-326 mimic; RT-qPCR, reverse transcription-quantitative polymerase chain reaction