Figure 5.

MiR-326 overexpression partially reversed the effect of CircHIPK3 on promoting BCa tumorigenicity in vivo. (a) Representative tumors from nude mice after injection of controls cells or MCF7 cells transfected with miR-326 and /or circHIPK3. (b) Tumor weights were measured. (c) Western blot detected the expressions of Bax, Bcl-2 and cleaved Caspase-3 in tumor from nude mice after the injection of controls cells or MCF7 cells transfected with miR-326 and /or circHIPK3. (d) RT qPCR determined the expressions of Bax, Bcl-2 in tumor from nude mice after the injection of controls cells or MCF7 cells transfected with miR-326 and /or circHIPK3. (e) RT-qPCR detected the expression of circHIPK3 in tumor from nude mice after the injection of controls cells or MCF7 cells transfected with miR-326 and /or circHIPK3. (f) RT-qPCR detected the expression of miR-326 in tumor from nude mice after the injection of controls cells or MCF7 cells transfected with miR-326 and /or circHIPK3. (g) Ki67 expression were determined by immunohistochemistry in each group. GAPDH was used as an internal control. *P < 0.05 or **P < 0.01 or ***P < 0.001 vs. NC+MC; ^#P < 0.05 or ^###P < 0.01 vs. CircHIPK3+ MC; ^{^^}P < 0.01 or ^{^^^}P < 0.001 vs. NC+M. Data are shown as mean±SD, n = 3. NC, negative control; MC, mimic control; M, miR-326 mimic; RT-qPCR, reverse transcription-quantitative polymerase chain reaction