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. 2021 Jun 7;20(13):1253–1269. doi: 10.1080/15384101.2021.1932029

Figure 2.

Figure 2.

Que inhibited the cell proliferation and miR-223-3p expression and regulated the expressions of proliferation-related and autophagy-related genes in ISO-reduced RCFs. (a). The cell proliferation of RCFs after induction by ISO or treatment with Que was detected by MTT assays. (b). The mRNA expressions of Cyclin D1, Fibronectin, and Col1A1 of RCFs after the induction with ISO or treatment with Que was detected by RT-qPCR, β-actin was used as an internal control. (c-d) The protein expressions of Cyclin D1, Fibronectin, and Col1A1 of RCFs after the induction with ISO or treatment with Que were detected by Western blot, β-actin was used as an internal control. (e) The expressions of miR-223-3p of RCFs after induction with ISO or treatment with Que were detected by RT-qPCR, U6 was used as an internal control. (f-i) The protein expressions of FOXO3, ATG7, LC3B-I, LC3B-II, and p62/SQSTM1 of RCFs after the induction with ISO or treatment with Que were detected by Western blot, β-actin was used as an internal control. All the experiments were conducted three times. (**P < 0.01, ***P < 0.001, vs. Control, ^^P < 0.01, ^^^P < 0.001, vs. ISO). (Que: quercetin, ISO: isoprenaline)