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. 2021 Jun 7;20(13):1253–1269. doi: 10.1080/15384101.2021.1932029

Figure 3.

Figure 3.

Que promoted autophagy in ISO-reduced RCFs. (a-b). The protein expressions of Cyclin D1, Fibronectin, and Col1A1of RCFs after the induction with ISO or 3-MA only, treatment with ISO and 3-MA, treatment with ISO and Que and treatment with ISO, Que and 3-MA were detected by Western blot, β-actin was used as an internal control. (c-e) The protein expressions of LC3B-I, LC3B-II, and p62/SQSTM1 of RCFs after the induction with ISO or 3-MA only, treatment with ISO and 3-MA, treatment with ISO and Que and treatment with ISO, Que and 3-MA were detected by Western blot, β-actin was used as an internal control. All the experiments were conducted three times. (***P < 0.001, vs. Control, ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, vs. ISO, ##P < 0.01, ###P < 0.001, vs. ISO+Que-H). (Que: quercetin, ISO: isoprenaline, 3-MA: 3-Methyladenine)