Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 2;10(1):1957215. doi: 10.1080/2162402X.2021.1957215

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Immune cell frequencies

a and b immunohistochemistry (IHC) based identification of myeloperoxidase (MPO) and CD14 expressing cells, respectively. A section from each tumor was stained for either MPO or CD14, images at 10x were taken from 5 different regions on each slide and the ratio of MPO/CD14 stain to the DAPI (nuclear stain) was determined using image analysis. Ratios (individual samples, and combined based on tumor grading) are shown in box plots. Images of three sections representing a low, medium and high ratio are shown as an inset in the graphs. Top panel of insets are images of IHC, and the bottom panel are digital conversions with pink representing the marker and blue nuclei for ease of viewing (enhanced contrast). In addition, percentages of CD15⁺ (neutrophils) and CD14⁺ (monocytes) cells determined by flow cytometry are shown below for comparison.c percentages of immune cell subsets in tumors determined via flow cytometry. Percentages are calculated as the proportion of each subset among total live cells. Cell types were determined using the following markers: CD45⁺ – all immune cells; CD15^high – neutrophils; CD15^med – other granulocytes (gran.); and CD14⁺CD15^neg and CD14⁺CD15^med as two monocyte subsets.d percentages of immune cell subsets in the blood. CD15^high – neutrophils and CD14⁺CD15^neg – monocytes. For statistical comparison of data, two-way ANOVA followed by Bonferroni’s test was performed. *** indicates p < .001. No significant difference was observed, if not indicated.