Figure 7.

MiR-590-5p inhibits cell growth and glycolysis in NSCLC via targeting METTL3. A549 and H1650 cells were transfected with miR-NC, miR-590-5p, miR-590-5p + pcDNA, or miR-590-5p + pcDNA-METTL3. (a-c) Cell proliferation analysis using CCK-8 and colony formation assays. (d) Flow cytometry assay of cell cycle. (e, f) Glucose uptake and lactate production detection in cells using commercial kits. (g) The glycolysis stress test of ECAR in cells. (h, i) Western blot analysis of CyclinD1, c-myc, GLUT1 and HK2 protein levels in cells. *P < 0.05