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. 2021 Jul 30;13(1):1946918. doi: 10.1080/19420862.2021.1946918

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© 2021 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — N49P7 and VRC07-523LS variants with improved potency and some HEp-2 reactivity. (a) Matrix of VRC01-class antibody variants. (b) Neutralization IC50 values of N49P7 variants and VRC07-523LS variants against a 12-virus panel. (c) HEp-2 cell staining assay against N49P7 and VRC07 variants was performed in the concentration of 25 μg/ml along with control antibodies, VRC01LS, 4E10, VRC07-523LS, and VRC07-G54W. Control antibodies were assigned a score between 0 and 3. Test antibodies scored greater than 1 at 25 µg/ml were considered polyreactive