Figure 5a.

Regulation of swollen conidia-induced PA production in A549 cells by CR3. (a-g) the intercellular PA content was measured by an enzyme-coupled fluorometric assay. A549 cells were stimulated with A. fumigatus ATCC13073 resting (a) or swollen (b) conidia opsonized with or without 10% human serum at an indicated MOI for 1 h. (c, d) A549 cells were infected by A. fumigatus ATCC13073 swollen conidia (MOI = 10) opsonized without (c) or with (d) 10% human serum for different duration. (e) The swollen conidia of A. fumigatus 13073 were opsonized with human serum, human serum plus C3 specific inhibitor compstatin (100 μM) or human serum plus control peptide (100 μM), then added into A549 cells at an MOI of 10 for 1 h