Table 1.
Discription of antibodies used in the study
| Destination | Cat. No. | Discription | Specificity | Applications | Source |
|---|---|---|---|---|---|
| Anti-CD11b | NBP1-28,423 | Mouse monoclonal [M1/70] | Human CD11b | Blocking | Novus |
| LM 2/1 | BMS104 | Mouse Monoclonal Antibody (LM2/1) | Human CD11b (I-domain) | Blocking | eBioscience |
| VIM 12 | CD11B00 | Mouse Monoclonal Antibody (VIM12) |
Human CD11b (Lectin-like domain) | Blocking | Invitrogen |
| Anti-CD11b | ab64347 | Rat monoclonal [M1/70] to CD11b | Human CD11b (I-domain) | Blocking | Abcam |
| IgG2 | 14–4724-82 | Mouse IgG2a kappa Isotype Control | Isotype control | Blocking | eBioscience |
| IgG1 | SA1-12,182 | Mouse IgG1 Isotype Control | Isotype control | Blocking | Thermo Scientific Pierce |
| Anti-CD11b | ab52478 | Rabbit monoclonal | Human CD11b (I-domain) | IHC-P, IP, WB | Abcam |
| Anti-CD18 | ab52920 | Rabbit monoclonal | CD18 phosphorylated on Serine 745 | WB | Abcam |
| Anti-iC3b | A209 | Mouse monoclonal | A murine monoclonal antibody to a neo-epitope expressed on iC3b | WB | Quidel |
| Anti-β-actin | sc-47,778 | Mouse monoclonal | Total levels of β-actin protein | WB | Santa Cruz |
| IgG | Zdr-5307 | HRP-conjugated goat anti-mouse | The secondary antibody | WB | ZSGB-BIO |
| IgG | Zdr-5306 | HRP-conjugated goat anti-rabbit | The secondary antibody | WB | ZSGB-BIO |
| Anti-CD11b | ab75476 | Rabbit monoclonal | Mouse CD11b aa 250–350 (internal sequence) | IF | Abcam |
| IgG | 1,400,412 | Alexa Fluor 594 goat anti-rabbit | The secondary antibody | IF | Life technologies |
| Anti-FAK | 3285 | Rabbit polyclonal | Endogenous levels of FAK protein | WB | CST |
| Anti-p-FAK | 3281 | Rabbit polyclonal | Endogenous levels of FAK only when phosphorylated at tyrosine 576/577 | WB | CST |
| Anti-Syk | 22,206-1-AP | Mouse polyclonal | Endogenous levels of Syk protein | WB | Proteintech |