Figure 3.

HvKP strains escape neutrophil-mediated phagocytosis and killing. (a) Neutrophil-like cell dHL-60 phagocytosis assay of HvKP1, HvKP2, cKP2, NP-HvKP, NP-HvKP/A, and NP-HvKP/A2. (b) Neutrophil killing assays of Klebsiella pneumoniae strains. (c) The resistance of K. pneumoniae strains to killing by pooled human serum. (d) Bacteria loads in blood, liver, lungs, kidney, and spleen were measured by plating at 24 hr post-infection. Bacterial load detectable in blood and various organs: HvKP1, cKP2, NP-HvKP/A and NP-HvKP. Data were analyzed by one-way ANOVA test. Each data point was repeated three times (n = 3). Data are presented as the mean ± s.e.m. * P < 0.05; ** P < 0.01; *** P < 0.001