Table 6.
Combined oral gavage and drinking water administration – protocols for antibiotic-induced gut depletion
| DURATION | ANTIBIOTIC COCKTAILS CONCENTRATIONS |
DISEASES (ICD 10) |
PUBLICATIONS |
|---|---|---|---|
| 5 consecutive days of gavage + 5 weeks in drinking water |
Oral gavage: ampicillin, neomycin, metronidazole and vancomycin for 5 days (0.2 mL: 10 mg of each antibiotic per mouse per day) Drinking water: ampicillin (1 g/L) + neomycin (1 g/L) + metronidazole: (1 g/L) + vancomycin: (0.5 g/L) for 5 weeks |
Endocrine, nutritional and metabolic diseases (Obesity) | 75 |
| 7 days |
Oral gavage: vancomycin (50 mg/kg) + neomycin (100 mg/kg) + metronidazole (100 mg/kg); Drinking water: Ampicillin (1 g/L) |
Disease of the circulatory system (Stoke, Seizure) | 76 |
| 14 days |
Oral gavage: vancomycin (5 g/L) + neomycin, (10 g/L) + metronidazole (10 g/L) + amphotericinB (0.1 g/L); Drinking water: ampicillin (1 g/l) + last 3 days: omeprazole via oral gavage (50 mg/kg, once a day) |
Disease of the nervous system (Epilepsy) | 77 |
| 14 days |
Oral gavage: 3 days of amphotericin-B (1 mg/kg BW) every 12 h + from day 3 Drinking water: ampicillin (1 g/L) + Oral gavage: every 12 h vancomycin (50 mg/kg BW) + neomycin (100 mg/kg BW) + metronidazole (100 mg/kg BW) + + amphotericin-B (1 mg/kg BW) |
Antibiotic-depletion protocol | 26 |
| 14 days |
Oral gavage: Vancomycin (250 mg) + neomycin-sulfate (500 mg) + ampicillin (500 mg) + metronidazole (500 mg) supplemented with 10 g grape Kool-Aid in 500 mL water + Driking water: Ampicillin (1 g/L) |
Disease of the respiratory system (Influenza) | 78 |
| 17 days |
Oral gavage: 3 days of amphotericin-B (1 mg/kg BW) every 12 h + from day 3 Drinking water: ampicillin ad libitum (1 g/L) + Oral gavage: every 12 h vancomycin (50 mg/kg BW) + neomycin (100 mg/kg BW) + metronidazol (100 mg/kg BW) + + amphotericin-B (1 mg/kg BW) |
FMT Protocol | 79 |