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. Author manuscript; available in PMC: 2021 Aug 3.
Published in final edited form as: Mol Microbiol. 2020 Apr 14;114(1):172–183. doi: 10.1111/mmi.14504

Fig. 2. Inactivation and complementation of dksA in B. burgdorferi.

Fig. 2.

(A) Schematic representation of the dksA locus in WT strain CE162, the mutant OY413, and the complemented strains OY463 and OY479. Genes are shown as thick arrows circumscribing the respective gene numbers. The small arrows indicate the approximate positions of the dksA promoter. (B, C) PCR (B) and RT-PCR (C) analyses of WT CE162, the dksA mutant, and the complemented strains. The specific target gene names are indicated on the right. Lane 1, WT CE162; lanes 2 and 3, two representative clones M1 and M2 of the mutant; lane 4, OY463; lane 5, OY479.