Fig. 5. DksA is involved in the tolerance of B. burgdorferi to osmotic stress.
B. burgdorferi was inoculated at 10,000 spirochetes per ml into modified BSK-II with 150 mM (A), 180 mM (B), or 200 mM (C) of NaCl. Spirochetes were enumerated daily by dark-field microscopy. Data are presented as the mean values ± standard deviation from three biological replicates. The asterisk indicates statistical significance (P < 0.05) between the mutants (M1 and M2) and WT CE162 or the complemented strains. WT, CE162; M1 and M2, two representative clones of the mutant; Com1, OY463; Com2, OY479.